Peak of Lanreotide not detected on Thermo HPLC

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Description:  Peak of Lanreotide not detected on HPLC of Thermo make.

Investigation: 

Investigation was done to find the root cause for above issue.

Mobile phase changed multiple times.

All HPLC tubings were checked for any kind of leakage but no issue was observed.

Root Cause :

To identify the root cause service engineer of Thermo was contacted.

He took multiple trails but not succeeded. 

After that he identifies that during method preparation calibration curve value taken was 5. As per his opinion it should be 6 for Thermo HPLC otherwise principle will not be detected.

The same method was previously run on waters make HPLC where calibration curve was 6 and the same wss latter included in method, but when run on Thermo make HPLC,  peak was not detected.

Conclusion: It is to be noted that calibration curve value play significant role during run on different HPLC systems. It is to be modified during development studies so that final method should include the correct value. 

If anybody is facing such issue then please check very first The calibration curve value and move ahead accordingly.


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