Hey!!! No peak found during HPLC Analysis

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Many times it happens that no peak is found during HPLC analysis. There are many such factors which gives rise to such situation. Out of that we will talk about 4 major factors today:

1) Solvent issue: 

Practically during analysis many times it happens that analyst uses wrong solvents for example instead of us in ACN they use Methanol. Due to this change in mobile phase, peak is not eluted. So very first it should be checked that correct solvent is used during HPLC analysis.

2) Detector Response: 

Second important factor is detector response. If there is any issue in detector response peak will not elute. Ensure that at the time if peak is not eluting then check the detector for any type of error.

3) Injector issue: 


Third major issue is with Injector. There can be any bubble entrapment which may give low response or no peak during HPLC analysis. Hence system should be purged properly to avoid such incidences.

4) Leak issue: 

Leak is also one of the most impacting parameter for no-peak issue. If there is any type of column leakage then it should be attended Immediately. This will save time and immediate rectification can be done.


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