3 impacting factors for peak broadning during HPLC analyais

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During HPLC analysis many times a issue is observed namely peak broadening. Due to this area increases abnormally giving rise to erroneous results. 

Some of the reasons and solutions for peak broadening are given below :

1) Temperature: Ambient temperature or room temperature can impact the broadening of peaks. To control it, column temperature should be kept in equilibrium mode using column heater or column cooler.
2) Equilibrium of column: Column and detector should be stabilized properly. During gradient system, sufficient time should be allowed between analyses and re-equilibriation. 
3) Incorrect column: Check that whether correct column is used during analyses with respect to dimensions as well as packing. If incorrect column is used it will give broad and improper peaks.


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