5 Major causes of noise in chromatograms during HPLC analysis?

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There are 5 major causes for too much noise during HPLC analysis?

Noise is the basic problem which every analyst faces during day to day HPLC analysis. Below are the causes which create noise and if properly handled, will definitely solve this problem.

1) Sampling rate: If too much noise is observed then very first check the sampling rate in HPLC method. It should be 1.0 instead of 10 (preferably in case of waters HPLC systems).

2) Pressure graph: Pressure graph to be monitored. If pressure fluctuations are more then it will give rose to noise creations during analysis.

3) Degassing: Degassing of mobile phase. If mobile phase is not degassed properly then it will take time to stabilize the column which will again generate the noise.

4) Detector: If room temperature is fluctuating too much then it will impact on detector which will disturb the baseline and will result in disturbed baseline. 

5) Column saturation: Some mobile phase requires some long time to saturate during HPLC analysis. So this will also result in noisy signals before analysis.

If all the points are handled properly then it will definitely solve the basic problem of noise which everybody encounters during HPLC analysis.

Upcoming Topic: 3 ways to get rid of %Carryover during HPLC analysis.


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